Journal: Biotechnology for biofuels and bioproducts

Article Title: Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate.

doi: 10.1186/s13068-023-02367-3

Figure Lengend Snippet: Fig. 8 Pulsed fed-batch cultivation of C. glutamicum ΔfasR cg2692TTG CgLP12::(Ptac-pntAB-TrrnB) gX (pEKEx2-maqu2220) with wheat straw hydrolysate. Fed-batch cultivations were conducted in a 1-L BioFlo120® bioreactor system with an initial batch volume of 0.5 L in CgXIImod medium supplemented with 195 µM PCA. The carbon source during the batch phase was provided by hydrolysate, normalized to a concentration of 40 g glucose L−1. 30 mL pulses (P) of a 350 g glucose L−1 hydrolysate stock solution were added three times upon a sharp increase of the DO signal. Shown process data are representative of three bioreactor cultivations using the same process conditions. Act acetate, DO dissolved oxygen, Glc glucose, X biomass, Xyl xylose

Article Snippet: Fed-batch cultivations were conducted in a 1 L BioFlo120® bioreactor system (Eppendorf SE, Hamburg, Germany) with an initial volume of 0.5 L. The cultivations were performed at 30 °C using an adjusted version of the CgXII medium to obtain high biomass concentrations (CgXIImod).

Techniques: Concentration Assay

Journal: Membranes

Article Title: The Effect of Heat Sterilization on Key Filtration Performance Parameters of a Commercial Polymeric (PVDF) Hollow-Fiber Ultrafiltration Membrane

doi: 10.3390/membranes12080725

Figure Lengend Snippet: A summary of hybrid biotechnological/membrane separation processes and the applied sterilization method. The dashes denote absence of relevant data.

Article Snippet: The handmade membrane modules were designed to be suitable for the head-plate and the height of a 3 L lab-scale bioreactor (BioFlo 120, Eppendorf, Hamburg, Germany).

Techniques: Membrane, In Situ, Serial Time-encoded Amplified Microscopy

Journal: bioRxiv

Article Title: Optogenetic Amplification Circuits for Light-Induced Metabolic Control

doi: 10.1101/2020.12.23.424152

Figure Lengend Snippet: (a) Schematic of 5-L fermentor setup with the dimensions of the area exposed to light. Red is the heating blanket around the reactor. Tan color depicts the cell culture (3 L). (b,c) Representative flow cytometry results using YEZ139, which has OptoEXP controlling GFP expression from P C120 (b) or YEZ336, which has OptoAMP4 controlling GFP expression from P GAL1-S (c). Cells were grown in the dark in batch using 15% glucose. When cultures reached an OD 600 of ~15, they were exposed to 5s ON / 95s OFF illumination for the rest of the experiment. Red histograms show fluorescence levels at pre-induction at an OD 600 of ~15; blue is after 3 h of induction at an OD 600 of ~23 (b) or ~22 (c); Purple is after 24 h of induction at an OD 600 of ~41. Grey is strain YEZ140 without GFP, which was used as a negative control. Each curve is generated from 10,000 single-cell events.

Article Snippet: To test OptoEXP or OptoAMP4 in higher cell density conditions, we inoculated YEZ139 (OptoEXP) or YEZ336 (OptoAMP4) into 10 mL of SC-His + 2% glucose from a single colony and grew in the dark for 16 h. We then set up a BioFlo120 system with a 5 L bioreactor (Eppendorf, B120110002) and added 3 L of SC-His medium supplemented with 15% glucose after autoclaving.

Techniques: Cell Culture, Flow Cytometry, Expressing, Fluorescence, Negative Control, Generated

Journal: Journal of biotechnology

Article Title: 3D printed autoclavable biocompatible biodegradable bioreactor vessels with integrated sparger made from poly-lactic acid.

doi: 10.1016/j.jbiotec.2024.06.001

Figure Lengend Snippet: Fig. 1. A) Simple bioreactor designed to be used in the Dasgip® Parallel Bioreactor System. B) Bioreactor vessel with the integrated zero dead-volume harvest line and sparger at the bottom of the reactor vessel. C) Detailed cut-out view of the integrated features on the bottom of the vessel.

Article Snippet: Batch cultivations were performed in a 1.5 L glass bioreactor with an L-sparger with a hole diameter of 0.5 mm (DS1500ODSS, 77102054, Eppendorf, Hamburg, Germany) and 3D printed bioreactor vessels with an integrated sparger with a hole diameter of 1.2 in the digital file and 0.8 measured in the final part.

Techniques:

Journal: Journal of biotechnology

Article Title: 3D printed autoclavable biocompatible biodegradable bioreactor vessels with integrated sparger made from poly-lactic acid.

doi: 10.1016/j.jbiotec.2024.06.001

Figure Lengend Snippet: Fig. 4. Bioreactor setup with the fully assembled 3D printed autoclaved bioreactor vessel (left) and glass vessel (right) set in place in the Das gip® Bioblock.

Article Snippet: Batch cultivations were performed in a 1.5 L glass bioreactor with an L-sparger with a hole diameter of 0.5 mm (DS1500ODSS, 77102054, Eppendorf, Hamburg, Germany) and 3D printed bioreactor vessels with an integrated sparger with a hole diameter of 1.2 in the digital file and 0.8 measured in the final part.

Techniques: